Apoptosis is induced by radiofrequency fields through the caspase-independent mitochondrial pathway in cortical neurons. - Université de Limoges Accéder directement au contenu
Article Dans Une Revue Radiation Research Année : 2008

Apoptosis is induced by radiofrequency fields through the caspase-independent mitochondrial pathway in cortical neurons.

Vanessa Joubert
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Sylvie Bourthoumieu
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Philippe Lévêque
OSA

Résumé

In the present study, we investigated whether continuous-wave (CW) radiofrequency (RF) fields induce neuron apoptosis in vitro. Rat primary neuronal cultures were exposed to a CW 900 MHz RF field with a specific absorption rate (SAR) of 2 W/kg for 24 h. During exposure, an increase of 2 degrees C was measured in the medium; control experiments with neurons exposed to 39 degrees C were then performed. Apoptosis was assessed by condensation of nuclei with 4',6-diamino-2-phenylindole (DAPI) staining observed with an epifluorescence microscope and fragmentation of DNA with TdT-mediated dUTP nick-end labeling (TUNEL) analyzed by flow cytometry. A statistically significant difference in the rate of apoptosis was found in the RF-field-exposed neurons compared to the sham-, 37 degrees C- and 39 degrees C-exposed neurons either 0 or 24 h after exposure using both methods. To assess whether the observed apoptosis was caspase-dependent or -independent, assays measuring caspase 3 activity and apoptosis-inducing factor (AIF) labeling were performed. No increase in the caspase 3 activity was found, whereas the percentage of AIF-positive nuclei in RF-field-exposed neurons was increased by three- to sevenfold compared to other conditions. Our results show that, under the experimental conditions used, exposure of primary rat neurons to CW RF fields may induce a caspase-independent pathway to apoptosis that involves AIF.
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Dates et versions

hal-00594011 , version 1 (18-05-2011)

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Vanessa Joubert, Sylvie Bourthoumieu, Philippe Lévêque, Catherine Yardin. Apoptosis is induced by radiofrequency fields through the caspase-independent mitochondrial pathway in cortical neurons.. Radiation Research, 2008, 169 (1), pp.38-45. ⟨10.1667/RR1077.1⟩. ⟨hal-00594011⟩
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