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Article Dans Une Revue Interventional Neuroradiology Année : 2023

A novel histological occlusion classification for coiled aneurysms based on multiphoton microscopy

Szatmary Zoltan
Cortese Jonathan
  • Fonction : Auteur
Mounier Jeremy
  • Fonction : Auteur
Perrin Marie-Laure
  • Fonction : Auteur
Janot Kevin
Couquet Claude
  • Fonction : Auteur
Aurélien Le Flahec
  • Fonction : Auteur
Leger-Bretou Claire
  • Fonction : Auteur
Mounayer Charbel
  • Fonction : Auteur
Rouchaud Aymeric
Sylvia M Bardet

Résumé

Objective Intracranial aneurysm (IA) coiling remains the most commonly used endovascular approach for ruptured and unruptured IA, and recanalization is a common drawback that impairs treatment success. Angiographic occlusion and aneurysm healing are not synonymous, and histological evaluation of embolized aneurysms remains a challenge. We propose here an experimental study of coil embolization in animal models by multiphoton microscopy (MPM) in comparison with conventional histological staining. The purpose of his work is to analyze coil healing process using histological sections of aneurysms. Methods Based on a rabbit elastase model, 27 aneurysms were fixed, embedded in resin, and cut in thin histological sections 1 month after coils implantation and after angiographic control. Hematoxylin and eosin (H&S) staining were realized. Non-stained adjacent slices were imaged for multiphoton excited autofluorescence (AF) and second-harmonic generation (SHG) to construct three-dimensional (3D) projections of sequentially and axially acquired images. Results The contrast provided by the combination of these two imaging modalities can be used to distinguish five levels of aneurysm healing, based on a combination of thrombus evolution and increased extracellular matrix (ECM) deposit. Conclusion RDPC:\Users\SHAHUL\RDP6|We have established a novel histological scale from a rabbit elastase aneurysm model after coiling with a classification of five different stages thanks to nonlinear microscopy. This classification is an actualized tool in order to obtain a more precise evaluation of occlusion device efficacy in the scope of new innovative microscopy for research.
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Dates et versions

hal-04413033 , version 1 (23-01-2024)

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Citer

Szatmary Zoltan, Cortese Jonathan, Mounier Jeremy, Perrin Marie-Laure, Janot Kevin, et al.. A novel histological occlusion classification for coiled aneurysms based on multiphoton microscopy. Interventional Neuroradiology, 2023, pp.159101992311579. ⟨10.1177/15910199231157926⟩. ⟨hal-04413033⟩

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