Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria - Université de Limoges
Article Dans Une Revue EMBO Molecular Medicine Année : 2024

Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria

Claire Kamaliddin
Bibiane Biokou
  • Fonction : Auteur
Jean-Eudes Degbelo
  • Fonction : Auteur
Latifou Dramane
  • Fonction : Auteur
Emilie Guillochon
  • Fonction : Auteur
Ludivine Houze
  • Fonction : Auteur
Sayeh Jafari-Guemouri
  • Fonction : Auteur
Sandrine Houze
Valentin Joste
  • Fonction : Auteur
Anaïs Labrunie
  • Fonction : Auteur
Yélé Ladipo
  • Fonction : Auteur
Thomas Lathiere
  • Fonction : Auteur
Achille Massougbodji
  • Fonction : Auteur
Audrey Mowendabeka
  • Fonction : Auteur
Jade Papin
  • Fonction : Auteur
Bernard Pipy
  • Fonction : Auteur
Marie Raymondeau
  • Fonction : Auteur
Jade Royo
  • Fonction : Auteur
Darius Sossou
  • Fonction : Auteur
Brigitte Techer
  • Fonction : Auteur
Bertin Vianou
  • Fonction : Auteur

Résumé

Abstract Cerebral malaria (CM), the most lethal complication of Plasmodium falciparum severe malaria (SM), remains fatal for 15–25% of affected children despite the availability of treatment. P. falciparum infects and multiplies in erythrocytes, contributing to anemia, parasite sequestration, and inflammation. An unbiased proteomic assessment of infected erythrocytes and plasma samples from 24 Beninese children was performed to study the complex mechanisms underlying CM. A significant down-regulation of proteins from the ubiquitin–proteasome pathway and an up-regulation of the erythroid precursor marker transferrin receptor protein 1 ( TFRC ) were associated with infected erythrocytes from CM patients. At the plasma level, the samples clustered according to clinical presentation. Significantly, increased levels of the 20S proteasome components were associated with SM. Targeted quantification assays confirmed these findings on a larger cohort ( n = 340). These findings suggest that parasites causing CM preferentially infect reticulocytes or erythroblasts and alter their maturation. Importantly, the host plasma proteome serves as a specific signature of SM and presents a remarkable opportunity for developing innovative diagnostic and prognostic biomarkers.

Dates et versions

hal-04527091 , version 1 (29-03-2024)

Identifiants

Citer

Jeremy Fraering, Virginie Salnot, Emilie-Fleur Gautier, Sem Ezinmegnon, Nicolas Argy, et al.. Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria. EMBO Molecular Medicine, 2024, 16 (2), pp.319-333. ⟨10.1038/s44321-023-00010-0⟩. ⟨hal-04527091⟩
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